LOCUS       sequence-57228-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT12BA
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgggcgaggt ggtcatgagc caagccatcc agccggcgca cgccactgcg
      121 cgcggcgagc tgagcgcggg gcagctgctc aagtggatcg acaccaccgc ctgcctggcg
      181 gctgagaaac atgctggagt ttcctgcgtt acagcctcag tggatgacat acagtttgag
      241 gagacagcta gagttggaca agttataacc atcaaagcaa aagttactag agcattcagc
      301 acaagcatgg agatcagtat caaggtcatg gtacaggata tgctcactgg cattgagaag
      361 cttgttagtg tggctttctc cacatttgta gccaaaccag ttggaaaaga aaagattcat
      421 ttaaaaccag tcacacttct aactgaacaa gatcatgtgg aacataatct ggctgctgag
      481 agaaggaaag ttcgattaca acatgaagat acctttaaca atttaatgaa ggaaagtagc
      541 aaatttgatg atctcatttt tgatgaagag gaaggagcgg tttccacaag gggcacctcc
      601 gttcagagca ttgaactggt cctcccaccc catgcaaacc atcacggaaa tacatttggt
      661 ggccagatta tggcgtggat ggagacagtg gctactattt ctgcaagccg cctgtgttgg
      721 gctcatccct ttctgaagtc cgtagatatg tttaagttcc ggggaccatc tacagttgga
      781 gatcgtcttg tcttcactgc cattgtcaac aatacatttc agacctgtgt tgaagttgga
      841 gttcgcgtgg aggcctttga ctgtcaggaa tgggccgagg gccgagggcg tcacatcaac
      901 agtgcttttc tcatttacaa tgctgctgat gataaggaaa atctcatcac gtttcccaga
      961 atccaaccca tttcaaagga tgatttcaga cgctatcggg gatgacagta aaggtggata
     1021 cggatccgaa
//
