LOCUS       sequence-57247-e         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLJ10324A
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaccccgc cgagctctcc acccagctgt cggcccctgg tgtcctgaag
      121 gtgtttgggg acagtgtctg cacaggaacc cactacaaga gcgtcctggc caccggcacc
      181 tccagcgccc gtgagctggt gaaggaggcg ctggagcggt acgccctgga ccccaggcag
      241 gccggccagt acgtgctgtg tgacgtggtg ggccaagccg gcgatgctgg gcagcggtgg
      301 caggcccggt gctttcgggt gtttggggac agtgagaagc ccctcttgat ccaggaatta
      361 tggaaacccc gagaaggttt atcccggagg tttgagctga ggaagaggtc ggacgtggag
      421 gagctggcag ccaaggaggt ggacaccatc acggcaggga taaacgccca ggcccggagg
      481 ctgcagcgga gtcgcgcgaa gtgacagtaa aggtggatac ggatccgaa
//
