LOCUS       sequence-57278-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX53A
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgacgtgtga tgacttgaaa agtggtgaaa agcgtctcat tccaaaacca
      121 acttgcaggt ttaaagacgc ttttcagcaa taccctgatc ttctgaaaag cataataagg
      181 gtagggattt taaagccaac gccaattcag tcacaggcat ggccaattat tctacaagga
      241 atagatctta tagtagttgc acaaaccgga acagggaaaa cattgtccta tctaatgcct
      301 gggtttattc atcttgattc tcaaccaata tctagagagc aaaggaatgg gcctgggatg
      361 ctagtcctta cacccactag agagttggct cttcacgtgg aagctgaatg ttcaaagtat
      421 tcatataaag gtctcaaaag catttgcata tatggtggta gaaacagaaa tggacaaata
      481 gaagacatta gcaaaggtgt agatatcatt attgcaactc ctgggaggct gaatgaccta
      541 caaatgaata actctgtcaa cctaagaagc ataacctact tggttataga tgaggcagat
      601 aaaatgctgg atatggaatt tgaaccccag ataaggaaga ttttattaga tgtgcgccca
      661 gaccgacaga ctgttatgac aagtgcaact tggccagata ctgtacgtca actagcactt
      721 tcttatttga aagatcctat gattgtttat gtttgacagt aaaggtggat acggatccga
      781 a
//
