LOCUS       sequence-57302-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CPSF7A
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaaccacc tcctcctgtt cgccaggagc catctcccaa gcccaacaac
      121 aagacccctg caattctgta tacctacagt ggcctgcgta atagacgagc tgccgtttat
      181 gtgggcagct tctcctggtg gaccacagac cagcagctga tccaggttat tcgctctata
      241 ggagtctatg atgtggtgga gttgaaattt gcagagaatc gagcaaatgg ccagtccaaa
      301 gggtatgctg aggtggtggt agcctctgaa aactctgtcc acaaattgtt ggaactccta
      361 ccagggaaag ttcttaatgg agaaaaagtg gacgtgaggc cggccacccg gcagaacctg
      421 tcacagtttg aggcacaggc tcggaaacgt gagtgtgtcc gagtcccaag atgacagtaa
      481 aggtggatac ggatccgaa
//
