LOCUS       sequence-57335-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(700..719)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 catatctcct gaagattttc attgttgtca gcttcctcca cccacttctg cagcaagggc
       61 cgcagcttac acatgttctt gaagctaagc tgcagagcct caaagcggca gatggtcgtt
      121 tggctgaata ccttcccaaa tagaaccccc agggtgagcc ccacatcggc ctgtgtatat
      181 cccagggtga tcctcttctg cttcaggagc ttggcaaatt gctcgagttc tttctgcaga
      241 gctttgatgt cctgggactc ctccgggttt tgctccagct tctccttctc cagcttcacg
      301 gcaccagggg tgacggtgca gggctccggg gaggccccat cggagttgct ctccaccccg
      361 actcctgctt cgccctcagg ctgagaggtc tccaagccgc cttggggcac tagccccact
      421 ccaacctggg gcccacagta cgccatcccc ccacagaact catacggcgg ggggcatggg
      481 ggaatccccc acacctcaga gcctggccca acccccggcc cgattcctgg ccctccagga
      541 gggccttgga agcttagcca ggtccgagga tcaacccagc ccggctccgg cccccctggc
      601 ccatcacctc caccacctgg agggggcgag aaggcgaaat ccgaagccag gtgtcccgcc
      661 atggtgaagg gcgaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc
      721 caggagctgt aggaaaaaga a
//
