LOCUS       sequence-57369-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse codon-optimized FLP recombinase with bovine 
            growth hormone polyadenylation (bpA) sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Raymond CS, Soriano P
  TITLE     High-efficiency FLP and PhiC31 site-specific recombination in 
            mammalian cells.
  JOURNAL   PLoS One. 2007 Jan 17;2(1):e162. doi: 10.1371/journal.pone.0000162.
  PUBMED    17225864
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2007-01-17"; volume: "2(1):e162. doi"; pages: " 
            10.1371/journal.pone.0000162"
COMMENT     Sequence Label: pPGKFLPobpA
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcttctatct ccgggccttt cgaccatcgc cgccaccatg gctcctaaga agaagaggaa
       61 ggtgatgagc cagttcgaca tcctgtgcaa gacccccccc aaggtgctgg tgcggcagtt
      121 cgtggagaga ttcgagaggc ccagcggcga gaagatcgcc agctgtgccg ccgagctgac
      181 ctacctgtgc tggatgatca cccacaacgg caccgccatc aagagggcca ccttcatgag
      241 ctacaacacc atcatcagca acagcctgag cttcgacatc gtgaacaaga gcctgcagtt
      301 caagtacaag acccagaagg ccaccatcct ggaggccagc ctgaagaagc tgatccccgc
      361 ctgggagttc accatcatcc cttacaacgg ccagaagcac cagagcgaca tcaccgacat
      421 cgtgtccagc ctgcagctgc agttcgagag cagcgaggag gccgacaagg gcaacagcca
      481 cagcaagaag atgctgaagg ccctgctgtc cgagggcgag agcatctggg agatcaccga
      541 gaagatcctg aacagcttcg agtacaccag caggttcacc aagaccaaga ccctgtacca
      601 gttcctgttc ctggccacat tcatcaactg cggcaggttc agcgacatca agaacgtgga
      661 ccccaagagc ttcaagctgg tgcagaacaa gtacctgggc gtgatcattc agtgcctggt
      721 gaccgagacc aagacaagcg tgtccaggca catctacttt ttcagcgcca gaggcaggat
      781 cgaccccctg
//
