LOCUS       sequence-57402-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a S527/799/1165A #31#907
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..164
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg ccaccatgga ctacaaagac catgacggtg
      121 attataaaga tcatgatatc gattacaagg atgacgatga caaagaattc aagttaaagg
      181 aagtagatcg tacagccatg caggcatgga gccctgccca gaatcacccc atttacctag
      241 caacaggaac atctgctcag caattggatg caacatttag tacgaatgct tcccttgaga
      301 tatttgaatt agacctctct gatccatcct tggatatgaa atcttgtgcc acattctcct
      361 cttctcacag gtaccacaag ttgatttggg ggccttataa aatggattcc aaaggagatg
      421 tctctggagt tctgattgca ggtggtgaaa atggaaatat tattctctat gatccttcta
      481 aaattatagc tggagacaag gaagttgtga ttgcccagaa tgacaagcat actggcccag
      541 tgagagcctt ggatgtgaac attttccaga ctaatctggt agcttctggt gctaatgaat
      601 ctgaaatcta catatgggat ctaaataatt ttgcaacccc aatgacacca ggagccaaaa
      661 cacagccgcc agaagatatc agctgcattg catggaacag acaagttcag catattttag
      721 catcagccag tcccagtggc cgggccactg tatgggatct taggaaaaat gagccaatca
      781 tcaaagtcag tgaccatagt aacagaatgc attgttctgg gttggcatgg catcctgatg
      841 ttgctactca gatggtcctt gcctccgagg atgaccggtt accagtgatc cagatgtggg
      901 atcttcgatt tgcttcctct c
//
