LOCUS       sequence-57504-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup 
            KD
  TITLE     Substantial energetic improvement with minimal structural 
            perturbation in a high affinity mutant antibody.
  JOURNAL   J Mol Biol. 2004 Oct 22;343(3):685-701.
  PUBMED    15465055
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2004-10-22"; volume: "343(3)"; pages: "685-701"
COMMENT     Sequence Label: pCT-4M5.3
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     93..116
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             447..458
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             459..485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc
       61 aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
      121 agaaaaaccc cggatcgaat tccctacttc atacattttc aattaagatg cagttacttc
      181 gctgtttttc aatattttct gttattgctt cagttttagc acaggaactg acaactatat
      241 gcgagcaaat cccctcacca actttagaat cgacgccgta ctctttgtca acgactacta
      301 ttttggccaa cgggaaggca atgcaaggag tttttgaata ttacaaatca gtaacgtttg
      361 tcagtaattg cggttctcac ccctcaacaa ctagcaaagg cagccccata aacacacagt
      421 atgtttttaa ggacaatagc tcgacgattg aaggtagata cccatacgac gttccagact
      481 acgctctgca ggctagtggt ggtggtggtt ctggtggtgg tggttctggt ggtggtggtt
      541 ctgctagcga cgtcgttatg actcaaacac cactatcact tcctgttagt ctaggtgatc
      601 aagcctccat ctcttgcaga tctagtcaga gcctcgtaca cagtaatgga aacacctatt
      661 tacgttggta cctgcagaag ccaggccagt ctccaaaggt cctgatctac aaagtttcca
      721 accgagtttc tggggtccca gacaggttca gtggcagtgg atcagggaca gatttcacac
      781 tcaagatcaa cagagtggag gctgaggatc tgggagttta tttctgctct c
//
