LOCUS       sequence-57531-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     Induction of cre recombinase activity using modified androgen 
            receptor ligand binding domains: a sensitive assay for 
            ligand-receptor interactions.
  JOURNAL   Nucleic Acids Res. 2003 Aug 1. 31(15):e86.
  PUBMED    12888538
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Aug 1. 31(15):e86."
COMMENT     Sequence Label: p259 pCMV-CRE-M (BglII)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    131..164
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     intron          386..518
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
       61 agaagacacc gactctagag gatcccctcg agcggccgcc taggccatgg cgaatttact
      121 gacggtacca ataacttcgt ataatgtata ctatacgaag ttattcgaat tccaaaattt
      181 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt
      241 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc
      301 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt
      361 tcgcgattat cttctatatc tgcaggtaag tatcaaggtt acaagacagg tttaaggaga
      421 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
      481 tggtcttact gacatccact ttgcctttct ctccacaggc gcgcggtctg gcagtaaaaa
      541 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
      601 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
      661 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
      721 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
      781 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
      841 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
      901 c
//
