LOCUS       sequence-57645-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green fluorescent genetically encoded Ca2+ 
            indicator for optical imaging (version 1.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..83
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     296..317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcggatccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca
       61 gagctatagg tcggctgagc tcactcgaga acgtctatat caaggccgac gagcagaaga
      121 acggcatcaa ggcgtacttc aagatccgcc acaacatcga gggcggcggc gtgcagctcg
      181 cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      241 actacctgag cgtgcagtcc atgctttcga aagaccccaa cgagaagcgc gatcacatgg
      301 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
      361 gcggtaccgg agggagcgaa tccatggtga gcaagggcga ggagctgttc accggggtgg
      421 tgcccatcca ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      481 agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      541 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      601 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      661 acatccagga gcgcaccatc ttcttcaagg gcgacggcaa ctacaagacc cgcgccgagg
      721 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      781 aggacggcaa catcctgggg cacaagctgg agtacaacac gcgtgaccaa ctgactgaag
      841 agcagatcgc agaatttaaa gaggctttct ccctatttga ca
//
