LOCUS       sequence-57725-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX210-U6-Pre-crRNA-Backbone-EF1a-hSpCas9
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgtgacgta cggccaccat ggactataag gaccacgacg gagactacaa ggatcatgat
       61 attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc
      121 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
      181 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
      241 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
      301 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
      361 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
      421 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
      481 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
      541 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
      601 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
      661 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
      721 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
      781 ctgagcaaga gcagacggct ggaaaatctg atcg
//
