LOCUS       sequence-57740-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Moss FJ, Imoukhuede PI, Scott K, Hu J, Jankowsky JL, Quick MW, 
            Lester HA
  TITLE     GABA transporter function, oligomerization state, and anchoring: 
            correlates with subcellularly resolved FRET.
  JOURNAL   J Gen Physiol. 2009 Dec;134(6):489-521.
  PUBMED    19948998
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2009-12"; volume: "134(6)"; pages: "489-521"
COMMENT     Sequence Label: pcDNA3.1(+)mGAT1-0-GFP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..730)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKI"
     primer_bind     complement(57..77)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     672..700
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     regulatory      727..736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacgggccct ctagattttg tatagttcat ccatgccatg tgtaatccca gcagctgtta
       61 caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt
      121 gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
      181 gttgataatg gtctgctagt tgaacgcctc catcttcaat gttgtgtcta gttttgaagt
      241 tagctttgat tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt
      301 tgtattccaa tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact
      361 cgattctatt aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc
      421 cgtcatcttt gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga
      481 aaaagtcatg ccgtttcata tgatctgggt atcttgaaaa gcattgaaca ccataagtga
      541 aagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa
      601 gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc
      661 cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc
      721 ctttacccat ggcggccgcc actgtgctgg atatctgcag aattcggatg taggcctcct
      781 tgctggctga gctgccagcc tgcggctgct cagggccatt ctcagggcgc acaatatctt
      841 cactgggctg aatcatgacc tggagacgct gcttcaggga gcccttcagg gtgaggaaca
      901 t
//
