LOCUS       sequence-57741-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Schirmer EC, Homann OR, Kowal AS, Lindquist S
  TITLE     Dominant gain-of-function mutations in Hsp104p reveal crucial roles 
            for the middle region.
  JOURNAL   Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20.
  PUBMED    14978213
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-05"; volume: "15(5)"; pages: "2061-72. Epub 2004 
            Feb 20"
COMMENT     Sequence Label: GAL-CDC12(WT)
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     11..27
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(58..388)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        271..712
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    271..388
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     683..706
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctt gaattttcaa
       61 aaattcttac tttttttttg gatggacgca aagaagttta ataatcatat tacatggcat
      121 taccaccata tacatatcca tatacatatc catatctaat cttacttata tgttgtggaa
      181 atgtaaagag ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata
      241 cgcttaactg ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc
      301 cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg
      361 cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat
      421 ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc
      481 aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt
      541 aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa
      601 ccactttaac taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata
      661 aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaa
//
