LOCUS       sequence-57996-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM
  TITLE     Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels
            to mTORC1.
  JOURNAL   Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
  PUBMED    22980980
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.07"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1196-208"
COMMENT     Sequence Label: pRK5-p18-HA
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(57..83)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      584..593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(646..665)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(678..696)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(679..696)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(906..930)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttatcatgcg
       61 tagtcaggca catcataggg gtaacctcca cctcctgcgg ccgctgggat cccaaactgt
      121 acaaccagct cctcttttgc gtccacacgg atctgagaaa gtgcactgta ggcataagca
      181 gctatcctgg agacctgctg caaatcagag aacgggatgg gctcactggc cagcacttgg
      241 tggggctggc tggtaagaga cggcagcggt ggcagcttct tccaatgggt caggctgctg
      301 ctcagcacag ccaagcgggt gctgtactgc ctggcacggt ccatgtactc atgctgctcc
      361 atgccctgtg agtctgcagc agacacatca atgatgttgc tggctgtctt ggcaaggatg
      421 gaagagagca gggcctgctc atcagtgcga gcggaaggca ggctgtggta gttgggctcg
      481 gctccattga gagctttggt aggggggctg ctagggtcca gcagcagctt ccgctcctct
      541 cggtcctggt ccgagtcctc gttctcgctg ctgtagcagc accccatggt ggcgtcgacg
      601 aattcaatcg atagaaccga ggtgcagttg gacctgggag tggacacctg tggagagaaa
      661 ggcaaagtgg atgttattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
      721 atgtattaat tgtagccgcg ttctaacgaa gccaaggggg tgggcctata gactctatag
      781 gcggtactta cgtcactctt ggcacgggga atccgcgttc caatgcaccg ttcccggccg
      841 cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc
      901 caggcgatct gacggttcac taaacgagct ctgcttatat agacctccca ccgta
//
