LOCUS       sequence-58009-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Azpiazu I, Gautam N
  TITLE     A fluorescence resonance energy transfer-based sensor indicates that
            receptor access to a G protein is unrestricted in a living mammalian
            cell.
  JOURNAL   J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12.
  PUBMED    15078878
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-06-25"; volume: "279(26)"; pages: "27709-18. 
            Epub 2004 Apr 12"
COMMENT     Sequence Label: alpha 0-CFP in pdest12.2
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     88..104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        127..145
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     127..144
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             220..231
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(550..578)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagccta
       61 ggccgcggga cggataacaa tttcacacag gaaacagcta tgaccattag gcctttgcaa
      121 aaagctattt aggtgacact atagaaggta cgcctgcagg taccggatca caagtttgta
      181 caaaaaagca ggcttcgaag gagatagaac caattcatta aagaggagaa attaaccatg
      241 ggatgtactc tgagcgcaga ggagagagcc gccctcgagc ggagcaaggc gattgagaaa
      301 aatctcaaag aagatggcat cagcgccgcc aaagacgtga aattactcct gctgggggct
      361 ggagaatcag gaaaaagcac cattgtgaag cagatgaaga tcatccatga agatggcttc
      421 tctggagaag acgtaaagca gtacaagcct gtcgtctaca gcaacaccat ccagtctctg
      481 gcagccattg tgcgggccat ggatactctg ggcggtacca tgagtaaagg agaagaactt
      541 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      601 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      661 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactactct cacttggggt
      721 gttcaatgct tttcaagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      781 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      841 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      901 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctat
//
