LOCUS       sequence-58189-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Voytas Golden Gate TALEN assembly 
            incorporating a CAG promoter for mammalian expression and a T7 
            promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain
            (ELD)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Hermann M, Cermak T, Voytas DF, Pelczar P
  TITLE     Mouse genome engineering using designer nucleases.
  JOURNAL   J Vis Exp. 2014 Apr 2;(86). doi: 10.3791/50930.
  PUBMED    24747757
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-04-2"; volume: "(86). doi"; pages: " 
            10.3791/50930"
COMMENT     Sequence Label: pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    769..790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(805..810,811..828,829..835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    843..859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgatctcgcc gttgttgaac ttgcgccgca cctcctccag tgtcagggtg ccggctttga
       61 tcatctcgcc gccgatcagc agctcctcca cgctcagcac ggcgccattg cagttggtga
      121 tgtggttcag cctggtcagc tgggccttgt agttgccctt gaagtggccg ctcacgaaca
      181 ggaacttgaa ctcggtcacg ctgctagggt acaccttcca ccactcgttg gggttgaggt
      241 gcttatcccg tgtctggttc tcctccacgt atctctccat ctcgtcggcc tggccgatag
      301 gcagattgta gccgccgctg taggcctttg tgtccacgat cacgccgtaa tcgatggggc
      361 tgcccactgt atagatggcg ccgtcaggct ttctgcttcc gcccaggtgc tctcccctgt
      421 agccgtacac cttcatgaag aactccatca ccttcatctc caggatgcgg tcctgggtgg
      481 ggttcctggc gatctcgatc agctcgatgt actcgtgggg cacgtacttc agcttgtgcc
      541 gcagctcgga cttcttctcc tccagctcgc tcttcaccag ctgggatccc gcaacgcgat
      601 gggacgtgcg ttcgccaata cggcgattga ctcttctgat caattccggc gcgtgcggca
      661 atcccttttt cactgcatcc atggcaggac gtccgccgag gcaggccaag gcgacgaggt
      721 ggtcgttgga gacgaccggt ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      781 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      841 aattgtgagc ggataacaa
//
