LOCUS       sequence-58204-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Kuhlman TE, Cox EC
  TITLE     Site-specific chromosomal integration of large synthetic constructs.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e92. Epub 2010 Jan 4.
  PUBMED    20047970
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6)"; pages: "e92. Epub 
            2010 Jan 4"
COMMENT     Sequence Label: pTKIP-dhfr
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..83
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             102..338
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     protein_bind    372..405
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca tcggcatagt
       61 atatcggcat agtataatac gacaaggtga ggaactaaac catgggtcaa agtagcgatg
      121 aagccaacgc tcccgttgca gggcagtttg cgcttcccct gagtgccacc tttggcttag
      181 gggatcgcgt acgcaagaaa tctggtgccg cttggcaggg tcaagtcgtc ggttggtatt
      241 gcacaaaact cactcctgaa ggctatgcgg tcgagtccga atcccaccca ggctcagtgc
      301 aaatttatcc tgtggctgca cttgaacgtg tggcctaatg aggggatcaa ttctctagag
      361 ctcgctgatc agaagttcct attctctaga aagtatagga acttcgatgg cgcctcatcc
      421 ctgaagccaa tagggataac agggtaatgc aggatgctgc tggctaccct gtggaacacc
      481 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
      541 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
      601 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
      661 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
      721 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
      781 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg
      841 cctcgcgcgt t
//
