LOCUS       sequence-58219-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD8L
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..180
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttaaggctag agtacttaat acgactcact ataggctagc ctcgagaatt gccaccatgg
       61 atatgagagt tcctgctcaa ttgctggggt tgctcttgct ctggtttcct ggcgaattca
      121 cgcgtggtac ctctagagtc gacccgggcg gccgcattgg aagttctgtt tcaagggcct
      181 gggagcgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg
      241 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag
      301 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac
      361 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc
      421 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag
      481 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa
      541 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg
      601 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc
      661 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg
      721 gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggc
//
