LOCUS       sequence-59143-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pBabe-Puro-RhoA FLARE.sc Biosensor
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(325..327,328..330,331..843)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(370..391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(631..650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccggccggat cccagtgtgg tggtacgtag gaattcatgg cacaccatca ccaccatcac
       61 ggtagtggca tcctggagga cctcaatatg ctctacatcc ggcagatggc actcagcctg
      121 gaggacacag agctgcagag gaaactagat catgagatcc ggatgaggga tggggcctgc
      181 aagctgctgg cagcctgctc ccagcgagag caggctctgg aagccaccaa gagcctgctg
      241 gtgtgcaaca gccgtattct cagctacatg ggtgagctgc agcggcgaaa ggaggcccag
      301 gtgctggaga agacaggcgg gggaatggtg agcaagggcg aggagctgtt caccggggtg
      361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaggttcag cgtgtccggc
      421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      601 tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      721 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
      781 atcaccgccg acaagcagaa gaacggcatc aaggcccact tcaagatccg ccacaacatc
      841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      901 cc
//
