LOCUS       sequence-59144-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pBabe-Puro-RhoA FLARE.sc Biosensor
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(346..597)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(388..409)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     737..756
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gatgatgggc acgttgggac agaaatgctt gacttctggg gtccactttt ctgggatgtt
       61 ttctaaacta tcagggctgt cgatggaaaa acacatcagt ataacatcgg tatctgggta
      121 ggagaggggc ctcaggcgat cataatcttc ctgcccagct gtgtcccaca aagccaactc
      181 tacctgcttt ccatccacct cgatatctgc cacatagttc tcaaacactg tgggcacata
      241 cacctctggg aactggtcct tgctgaagac tatgagcaag catgtctttc cacaggctcc
      301 atcaccaaca atcaccagtt tcttccggat ggcagccatt ccccccttgt acagctcgtc
      361 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      421 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      481 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      541 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      601 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      661 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      721 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      781 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      841 gcgggcgaag cacatcaggc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg
      901 cagcttgccg gtggtgcaga tgaacttcag ggtca
//
