LOCUS       sequence-59280-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPKCA
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcct
      121 tgggtccagc tttctggaca tgctgggaac ttccaggcag gagaggatgg tcggattctg
      181 aaacgtttct gtcagtgtga gcagcgcagc ctggagcagc tgatgaaaga cccgctgcga
      241 cctttcgtgc ctgcctacta tggcatggtg ctgcaggatg gccagacctt caaccagatg
      301 gaagacctcc tggctgactt tgagggcccc tccattatgg actgcaagat gggcagcagg
      361 acctatctgg aagaggagct agtgaaggca cgggaacgtc cccgtccccg gaaggacatg
      421 tatgagaaga tggtggctgt ggaccctggg gcccctaccc ctgaggagca tgcccagggt
      481 gcagtcacca agccccgcta catgcagtgg agggaaacca tgagctccac ctctaccctg
      541 ggcttccgga tcgagggcat caagaaggca gatgggacct gtaacaccaa cttcaagaag
      601 acgcaggcac tggagcaggt gacaaaagtg ctggaggact tcgtggatgg agaccacgtc
      661 atcctgcaaa agtacgtggc atgcctagaa gaacttcgtg aagctctgga gatctccccc
      721 ttcttcaaga cccacgaggt ggtaggcagc tccctcctct tcgtgcacga ccacaccggc
      781 ctggccaagg tctggatgat agacttcggc aagacggtgg ccttgcccga ccaccagacg
      841 ctcagccaca ggctgccctg ggctgagggc aaccgtgagg acggctacct ctggggcctg
      901 gacaacatga tctgcctcct gcaggggctg gcacagagct gataacagta aaggtggata
      961 cg
//
