LOCUS       sequence-59296-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CPSF5A
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             803..820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(873..891)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgggca
      121 acaagtacat ccagcagacg aagcccctca ccctggagcg caccatcaac ctgtaccctc
      181 ttaccaatta tacttttggt acaaaagagc ccctctacga gaaggacagc tctgttgcag
      241 ccagatttca gcgcatgagg gaagaatttg ataaaattgg aatgaggagg actgtagaag
      301 gggttctgat tgtacatgag caccggctac cccatgtgtt actgctgcag ctgggaacaa
      361 ctttcttcaa actacctggt ggtgaactta acccaggaga agatgaagtt gaaggactaa
      421 aacgcttaat gacagagata ctgggtcgtc aggatggagt tttgcaagac tgggtcattg
      481 acgattgcat tggtaactgg tggagaccaa attttgaacc tcctcagtat ccatatattc
      541 ctgcacatat tacaaagcct aaggaacata agaagttgtt tctggttcag cttcaagaaa
      601 aagccttgtt tgcagtccct aaaaattaca agctggtagc tgcaccattg tttgaattgt
      661 atgacaatgc accaggatat ggacccatca tttctagtct ccctcagctg ttgagcaggt
      721 tcaattttat ttacaactga cagtaaaggt ggatacggat ccgaattcga gctccgtcga
      781 caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      841 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      901 tggggcctct aaacgggtct tgagggtttt tgctgaagga gaactatatc cggattgc
//
