LOCUS       sequence-59314-e        6328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter 
            MT, Watnick T, Weimbs T
  TITLE     Polycystin-1 regulates STAT activity by a dual mechanism.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr
            25.
  PUBMED    21518865
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-05-10"; volume: "108(19)"; pages: 
            "7985-90. Epub 2011 Apr 25"
COMMENT     Sequence Label: CTM-PC1
FEATURES             Location/Qualifiers
     source          1..6328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1234..1263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1273..1302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1318..1335
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1358..1375)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1364..1588
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1634..2062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1721..1740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1931..1952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2071..2091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2076..2406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2257..2392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2319..2338
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2454..2501
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2520..2894
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3024..3145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3061..3080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3115..3134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3194..3210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3194..3210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3207..3229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3218..3234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3242..3248,3249..3266,3267..3272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3287..3308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3425..3442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3596..4184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3676..3695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4355..5146,5147..5215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4978..4997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5216..5320)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5395..5414)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5586..5965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5966..6169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6120..6140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6171..6189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6192..6210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6220..6244
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aagcttctag agatccctcg acctcgagat ccattgtgct ctaaagatct ttggtgactt
       61 gtccactcca gtgtggcatc atgtggcagc tgctcctccc aactgctctg ctacttctag
      121 tttcagctgg catgcggact gaagatctcc caaaggctgt ggtgttcctg gagcctcaat
      181 ggtacagcgt gcttgagaag gacagtgtga ctctgaagtg ccagggagcc tactcccctg
      241 aggacaattc cacacagtgg tttcacaatg agagcctcat ctcaagccag gcctcgagct
      301 acttcattga cgctgccaca gtcaacgaca gtggagagta caggtgccag acaaacctct
      361 ccaccctcag tgacccggtg cagctagaag tccatatcgg ctggctgttg ctccaggccc
      421 ctcggtgggt gttcaaggag gaagacccta ttcacctgag gtgtcacagc tggaagaaca
      481 ctgctctgca taaggtcaca tatttacaga atggcaaaga caggaagtat tttcatcata
      541 attctgactt ccacattcca aaagccacac tcaaagatag cggctcctac ttctgcaggg
      601 ggcttgttgg gagtaaaaat gtgtcttcag agactgtgaa catcaccatc actcaaggtt
      661 tggcagtgtc aaccatctca tcattctctc cacctggcgc ggatccacca agggcctctg
      721 ccctccctgc cccaccgaca ggctccgccc tccctgaccc gcagacagcc tctgccctcc
      781 ctgacccgcc agcagcctct gccctccctg cggccctggc ggtgatctcc ttcctcctcg
      841 ggctgggcct gggggtggcg tgtgtgctgg cgaggacgcg tagccagctg gatgggctga
      901 gcgtgagcct gggccggctg gggacaaggt gtgagcctga gccctcccgc ctccaagccg
      961 tgttcgaggc cctgctcacc cagtttgacc gactcaacca ggccacagag gacgtctacc
     1021 agctggagca gcagctgcac agcctgcaag gccgcaggag cagccgggcg cccgccggat
     1081 cttcccgtgg cccatccccg ggcctgcggc cagcactgcc cagccgcctt gcccgggcca
     1141 gtcggggtgt ggacctggcc actggcccca gcaggacacc ccttcgggcc aagaacaagg
     1201 tccaccccag cagcacttag ggcggccgct cgagaacaaa aactcatctc agaagaggat
     1261 ctggggccct tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat
     1321 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     1381 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1441 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1501 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1561 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     1621 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1681 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1741 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     1801 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1861 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1921 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1981 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     2041 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2101 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2161 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2341 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2401 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     2461 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     2521 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     2581 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     2641 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     2701 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     2761 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     2821 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     2881 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     2941 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3001 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3061 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3121 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3181 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3241 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3301 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3361 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3421 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3481 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3781 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4021 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5341 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat
     5401 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     5461 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     5521 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     5581 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     5881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     5941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     6001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6061 gttttggaac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
     6181 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
     6241 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     6301 cagcctccgg actctagcgt ttaaactt
//
