LOCUS       sequence-59340-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: p156RRL-EF1a-GFP-U3H1SatA
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(340..373)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             393..573
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(598..616)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cccgtgtcca acgaaatcct caaaggtatc aaaatatcca cttgcagatt ctacaaaaag
       61 agtgcttcaa aactgctctg tcaaaaggaa ggttcaactc tgttacttga gtacacacat
      121 cacaaggaag tttctgagaa tgctgctaga tctgtctagt tttcatatga agatatcccg
      181 tgtccaacga aatcctcaaa ggtatcaaaa tatccacttg cagattctac aaaaagatgt
      241 ttccaaactg ctgtatcaat agacaggttg tactctgtta gttgaggaca cacatcaaaa
      301 agaagtttct gagaatgcta tcgataccgt cgcatgggaa taacttcgta tagcatacat
      361 tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
      421 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      481 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      541 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
      601 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
      661 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
      721 atcatctctc tcgtaaactg ccgtcagagt cggttt
//
