LOCUS       sequence-59389-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TLR10A
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             646..663
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(716..734)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      730..777
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctaga aataattttg tttaacttta agaaggagat atacatatgc accatcatca
       61 tcatcattct tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaaaac
      121 aacccaagaa caactcaaga gaaatgtccg attccacgca tttatttcat acagtgaaca
      181 tgattctctg tgggtgaaga atgaattgat ccccaatcta gagaaggaag atggttctat
      241 cttgatttgc ctttatgaaa gctactttga ccctggcaaa agcattagtg aaaatattgt
      301 aagcttcatt gagaaaagct ataagtccat ctttgttttg tctcccaact ttgtccagaa
      361 tgagtggtgc cattatgaat tctactttgc ccaccacaat ctcttccatg aaaattctga
      421 tcatataatt cttatcttac tggaacccat tccattctat tgcattccca ccaggtatca
      481 taaactgaaa gctctcctgg aaaaaaaagc atacttggaa tggcccaagg ataggcgtaa
      541 atgtgggctt ttctgggcaa accttcgagc tgctattaat tgacagtaaa ggtggatacg
      601 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      661 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      721 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      781 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc cctgtagcgg cgcattaagc
      841 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      901 gctcctttcg ctttcttcct tctttctcgc acgtcg
//
