LOCUS       sequence-59396-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPK1A
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcag
      121 acctttctga aagggaagag agttggctac tggctgagcg agaagaaaat caagaagctg
      181 aatttccagg ccttcgccga gctgtgcagg aagcgaggga tggaggttgt gcagctgaac
      241 cttagccggc cgatcgagga gcagggcccc ctggacgtca tcatccacaa gctgactgac
      301 gtcatccttg aagccgacca gaatgatagc cagtccctgg agctggtgca caggttccag
      361 gagtacatcg atgcccaccc tgagaccatc gtcctggacc cgctccctgc catcagaacc
      421 ctgcttgacc gctccaagtc ctatgagctc atccggaaga ttgaggccta catggaagac
      481 gacaggatct gctcgccacc cttcatggag ctcacgagcc tgtgcgggga tgacaccatg
      541 cggctgctgg agaagaacgg cttgactttc ccattcattt gcaaaaccag agtggctcat
      601 ggcaccaact ctcacgagat ggctatcgtg ttcaaccagg agggcctgaa cgccatccag
      661 ccaccctgcg tggtccagaa tttcatcaac cacaacgccg tcctgtacaa ggtgttcgtg
      721 gttggcgagt cctacaccgt ggtccagagg ccctcactca agaacttctc cgcaggcaca
      781 tcagaccgtg agtccatctt cttcaacagc cacaacgtgt caaagccgga gtcgtcatcg
      841 gtcctgacgg agctggacaa gatcgagggc gtgttcgagc ggccgagcga cgaggtcatc
      901 cgggagctct cccgggccct gcggcaggca ctgggcgtgt cactcttcgg catcgac
//
