LOCUS       sequence-59397-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAK1A
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             692..709
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(762..780)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      776..823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgtctg
      121 cttctgagga gcaggtagcc caggacacag aggaggtttt ccgcagctac gttttttacc
      181 gccatcagca ggaacaggag gctgaagggg tggctgcccc tgccgaccca gagatggtca
      241 ccttacctct gcaacctagc agcaccatgg ggcaggtggg acggcagctc gccatcatcg
      301 gggacgacat caaccgacgc tatgactcag agttccagac catgttgcag cacctgcagc
      361 ccacggcaga gaatgcctat gagtacttca ccaagattgc caccagcctg tttgagagtg
      421 gcatcaattg gggccgtgtg gtggctcttc tgggcttcgg ctaccgtctg gccctacacg
      481 tctaccagca tggcctgact ggcttcctag gccaggtgac ccgcttcgtg gtcgacttca
      541 tgctgcatca ctgcattgcc cggtggattg cacagagggg tggctgggtg gcagccctga
      601 acttgggcaa tggtcccatc ctgaactgac agtaaaggtg gatacggatc cgaattcgag
      661 ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      721 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      781 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      841 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      901 gttacgcgca gcgtgaccgc tacacttgcc agcgcc
//
