LOCUS       sequence-59422-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX20A
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             867..884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgg
      121 accgctcagg atctcagcag cccgcggacc cgcacggggg atgtgctgtt ggcggagccg
      181 gccgacttcg agtcactgct gctttcgcgg ccggtgctgg aggggctgcg ggcggccggc
      241 ttcgagaggc cctcgccggt gcagctcaag gccatcccgc tggggcgctg cgggctcgat
      301 ttaattgttc aagctaaatc tggcaccggg aaaacctgtg tgttctccac catagctttg
      361 gactctcttg ttcttgaaaa cttaagtacc cagattttga tcttggctcc tacaagagaa
      421 attgctgtac agatacattc tgttattaca gccattggaa taaaaatgga aggcttagag
      481 tgtcatgtct ttattggagg gaccccatta tcacaagaca aaaccagact taaaaagtgt
      541 catattgctg ttggatctcc tggcagaatt aagcaactca tagaacttga ctacttgaac
      601 ccaggcagta tacgcctctt tattcttgat gaagcagata agcttttaga agaaggcagc
      661 ttccaggagc aaataaattg gatttattct tccttgcctg ccagtaaaca gatgctggca
      721 gtatcagcta cttatcccga atttttggct aatgctttga caaagtacat gagagatccc
      781 acttttgtaa gactgaattc ctgacagtaa aggtggatac ggatccgaat tcgagctccg
      841 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      901 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaata
//
