LOCUS       sequence-59551-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6B-(997-1084)-NES-mut
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(332..349)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    338..562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      608..988
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(695..714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     905..926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gacgagctgt acaagtccgg cggcaagctt ctcaaaggag gaaacaatga ttcatggatg
       61 aatcctgccg ccaaacaggc ttcaaatgcc ggagctctga gtcagactga agataatcca
      121 agcagcaaaa tggatttgtc tgtaggaagc ctttcagata aaaaatttga tgtggacaag
      181 cgagcgatga atctcgggga ttttaatgat atcatgagga aggatcgatc tgggttccgt
      241 ccacctaatt ccaaagacat gggaaccaca gatagtgggc cttattttga gaagggctga
      301 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      361 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      421 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      481 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
      541 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
      601 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      661 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      721 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      781 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      841 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      901 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      961 gatttataag ggattttgcc gatttcggcc tattggttaa aaa
//
