LOCUS       sequence-59568-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T
  TITLE     Arl13b regulates ciliogenesis and the dynamic localization of Shh 
            signaling proteins.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.
  PUBMED    21976698
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2011-12"; volume: "22(23)"; pages: "4694-703. Epub 
            2011 Oct 5"
COMMENT     Sequence Label: pDEST47-Arl13b17
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             693..713
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(725..749)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(781..800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(782..800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(845..896)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(874..894)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac tttgtacaag
       61 aaagctgggt cggcgcgccc acccttttca cttaaggcat caaagtcctt tgcaatgata
      121 tgcagtagcc aataaagccc ctttttaatg gacttgtcaa ttttctttcc atatcccaag
      181 actgctgaac aaggttcgat ctgacacagg cacttgtgct cgttgaccag cttctccagc
      241 gagagacact caatcacatc cgcttcgcct aaagccccct ccttgtcttg cttgttggcc
      301 agcaccaata taggctttcc tgatatcctc gggtgtctta gcacttcgga cattgtctcc
      361 tttgtttctt ccattctctc ctcatcactg gaatccacaa caaatattac cccataggat
      421 tcagcataat aattcttcca aattccccga attctttttc cacctcctaa gtcaaagatg
      481 gtaacttgga actttccttg tctcagatca attttagaaa agccaacagt gggagctacg
      541 tcttcaggat gctctccttg gattcccttt gctgtggctg ttttaccagc attatcaagg
      601 ccgaccatca caagtgtcac ctttctgaca ggctcccgcc accgcttgaa caagttgcag
      661 cagttggcca tcagactgaa catggtgaag ggctccttct taaagttaaa caaggcggcc
      721 gcggagcctg cttttttgta caaacttgtt tgatcaagct taactagcca gcttgggtct
      781 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      841 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      901 gggcggagtt gttacgacat tttggaaagt cccgttga
//
