LOCUS       sequence-59659-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: Enhanced Repressor Domain for TAL Effector (SI...
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(438..458)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(529..545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(529..545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(542..564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    553..569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(577..583,584..601,602..607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    622..643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(760..777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtacctcgc gaatgcatct agatcatcca attctagatc tactgggcag catagaggca
       61 tagccatgtt cagcttctct ctcgcgcctc tccaggtaat cggcagcctc cagcagcatt
      121 tgtatgttca tcccgctgcc tggcagcatg ctggcgtatc cgtgctcggc ttctctttct
      181 ctccgttcca ggtaatcggc tgcctccagc agcatttgga tattcattcc agacccaggc
      241 agcatggaag catagccatg ttcggcctcc cgttcgcgcc tttccaggta atcagcagcc
      301 tccagcagca tctgaatatt catgccgctt ccgggcagca ttgaagcata tccgtgctcg
      361 gcttcgcgct ctctccgttc caggtagtca gcggcctcca gcagcatctg gatgttcatc
      421 ccggagccgc tggcctccac cttcctcttt ttcttggggc tagcatgatt gatcggatcc
      481 cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt
      541 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      601 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      661 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      721 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      781 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      841 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      961 tcacaa
//
