LOCUS       sequence-59682-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt1-V5
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccagaca ctcgtgcagt acgcgcgtgt gcgtgcagtt gcggcagctg acgtggcagc
      121 accagtggaa ggtgcagttg cagcgctcgg tgacgcgctg cgtgcgcgtg cggtggcccc
      181 tgccgcagca gagcagctcg cagccgtcca gcgcgggcga cgagctgtta caggcgcgcc
      241 ctgccgtgcc tgctgtgccc aggcgtccgc tgtacgtgca gaagttgggc gatttctcga
      301 agtagacgag gtcgtggggg gagggcggtt tgtgggccgg gtcttccggc tccaggcgca
      361 gcagctccgc ccgcgaagcg cggttgctgc cgcggttgcc gtacaggacg cgcgaggcgc
      421 cgtcgaagcg gtcgcgcagc acatcgccca cggcgcgcag cgtgggcagc cgcatccagc
      481 acgtgcgcac cgtgcatgag ccggacatcc cgtggcactt gcactcctgg cgcatctcgg
      541 agaatacggt cgtacggcct gcctcgttgt tgtgaaggtt catgaggaag cgcaggtccc
      601 gccccttctc cccggagtcc acgaactccc ggccgaagag gcggccgaag tcaatgttgt
      661 cgctgcagcc cccccagtgc cagtcggggc ccccggggcc gcgccgccgg tagtcacacg
      721 tgcaggattc gatggaacct tctgagcagg agcgcgccac cgaatgggtg accccggcgg
      781 aggtgatagc gaagataaac gccgtttctc gacagcctcg gttgacgatc ttgccgaaga
//
