LOCUS       sequence-59684-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt2-V5
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 ctccgcctgt agcggttgtc cagtcagcgt tcttgggggc cttgcatgtg tgcacatcca
      121 gagcttccag gcagtcctga cagcgcacgg cgcagcacca gtggaactta cacccacact
      181 tggtcatccg ggtgacatgg gaggtgtcgt agcctctccc acagcacatg acttcacagc
      241 tgtccatgcc ccgggaagtc aggttgcaca cacggcctgc tgtacccagg gagcctgcct
      301 ctcggtccct gatacagtag tctggagaat tttcaaaata cacgaggtca tttttcgttg
      361 gcttcttaaa cctctcgtta gccacagtga aacctgtgcc atcctggttc atgaccacct
      421 ggatggcccc attgtacttc ctccagagat aatcgcccgt tttcctgaag tcggccatgg
      481 ccagccagca tgtcctgaga gtacatgagc cgctcacccc gtggcacttg cactcttgtt
      541 tcaagaaccg ctttacagcc ttcctgccag ctctgttgtt gtgaagattc atcagggctc
      601 tggcatcctt tcctttcctt tcctttgcat ccacaaatgc gcgggcaaat ttgatcccat
      661 agtcaatgtt atcactgcag ccaccccaat caaaaatgcc tttgctgtcc ttggcgcttc
      721 ccatcttctt tggatcacag gaacaggatt ttacttctcc ttggctacag gccctggtga
      781 tggcaaatac aactccagct gaggagatgg cataaacaaa ggcagattcc cgactacttc
      841 ggagtaggac cctgccaaaa aggctgtgat ccctgtccag ggtgttgcaa ttccagc
//
