LOCUS       sequence-59686-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt3-V5
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca ggtgtgcacg tcgtagatgc gaatacactc ctggcagctg acgtagcagc
      121 accagtggaa gatgcagtgg catttttcct tccgcttctc cgtcctcgtg ttgtggcccc
      181 ggccacagca gagcagatcg cagccatcga tgccgtggga ggtgacattg caagtccggt
      241 cccttgtgcc aaaggaaccc gtctctgggt tgggctcaca aaagttgggg gagttctcgt
      301 agtagaccag gtccctctcc gtgggtggct tgaagagcga gtacttggcc cggagggtct
      361 ccacccagcc tcgggactca cggtgcttct ctactaccat ctccgaggcg ctgtcatact
      421 tgtccttgag gaagtcaccg atggcacgga agtcaggctg cgcccaccag caggtcttca
      481 cctcacagct gcccgacagc ccgtggcact tgcatttgag gtgcatgtgg tccaggatag
      541 tcgtgcggcc cgcctcgttg ttgtgcttgt tcatggccga gcgcgcgtcc ggcctgttct
      601 cgcgcgcatc cgcgaactcc ctggacacta acacgccgaa gtcagcgtcc tcgctgcagc
      661 cgccccactt ccagccttcg ccaggcggcc ccttatgatg cgagtcacag ccgcaaatgg
      721 tggaggtgcc ctcggcgcag gagcgggtga cggcgaaggc cacgccggcc ga
//
