LOCUS       sequence-59689-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: psiCHECK-CXCR4 target
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    265..313
                     /note="synthetic polyadenylation signal"
     promoter        346..450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             635..1013
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(669..688)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg
       61 gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg
      121 aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga
      181 acgagcagta attctaggcg atcgctcgag aagttttcac aaagctaaca gaattcgttt
      241 aaacctagag cggccgctgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
      301 ggttttttgt gtgaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      421 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      481 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      541 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      601 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      661 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      721 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      781 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      841 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      901 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      961 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1021 ctattaactg gcgaactact tactctagct t
//
