LOCUS       sequence-59690-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt5A-V5
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca cacaaactgg tccacgatct ccgtgcactt cttgcacttg acgtagcagc
      121 accagtggaa cttgcagtgg cagcgctccg tctgcacggt cttgaactgg tcgtagccac
      181 ggccgcagca catgagctcg cagccatcca tgccctccga cgtcttgttg cacaggcggc
      241 cctgcgtgcc cagcgagccg gtgctctcat tgcgcacgca gtagtcaggg ctggggtcga
      301 tgtagaccag gtcttgtgtg gtgggcgagt tgaagcggct gttgacctgt accaacttgc
      361 cccggctgtt gagccgcatg gccgccgcgc tgtcgtactt ctccttcagg gcatcaccca
      421 ccttgcggaa gtctgccagc tgcagccagc atgtcttcag gctacatgag ccggacaccc
      481 catggcactt gcaggccaca tcagccaggt tgtacaccgt cctgcggccg gcctcgttgt
      541 tgtgcaggtt catgaggatg cgagcactct cgtaggagcc cttggcgtgg atgcgctccc
      601 gctcgcgggc gtccacgaac tccttggcaa agcggtagcc atagtcgatg ttgtcgccgc
      661 agccgcccca gagccagtcc cgcggcaggt ccttggggcg cgcggcgcgg ctgcagccgc
      721 aggtggacag ctcgccctcg cggcacgccc ggctcatggc gttcaccacc cctgctgcgc
      781 tcaccgcgta tgtgaaggcc gtctcgcggc tgcctatctg catcaccctg ccaaaaacag
      841 aggtgttatc cacagtgctg cagttccacc ttcgatgtcg gaa
//
