LOCUS       sequence-59700-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt10A-V5
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca gacgctgacc cactcggtga tgcggcactc ttcgcagacc acgaaacagc
      121 accagtggaa gcggcagtgg cagcgctcgc tgcgcgtctg gcgcaggatg ttgtggccgc
      181 ggccgcagca catgctgccg cagccatccg agccggcgct gctcttgttg cacaggcggc
      241 ccacggtgcc cgccgagtcc aggcgcggct cgcgctcgca gaagtcggga gacttttcga
      301 agtagaccag gtcggcgggg ctggcccgtc ggcgcggccc gggagcgccc ggagccggcg
      361 agggtgcccc cgctgggccc ggctccagct ggccgccgtt gcggttgtgc ggccggatga
      421 gcgtggcgcg gtggaagcgg ctgcgcagca gcgcccccac ggtgcggaac tcgggcgtca
      481 cctgccagca cgtcttgagc tggcagctgc ctgacgtgcc gtggcacttg cacttccgcc
      541 gcatgttctc catcactgcc tgcctcccaa ctcggttgtt gtgaagcctc attctcgcgt
      601 ggatgtctct gtgaggctcc cgggagtcca gaaagtcctt agaaaagcgc tccccgaagc
      661 ccatgtcggg gctgcagccg ccccactccc aggagtcctg caggcctggg ctggctgtgg
      721 gcagggctgg gtgttccggg accccatggc tcaggccctt accacgctgc agtgcatcca
      781 gttgtaagcg gtgcagcttc ctacggaagg cctcctcgtc ccctcgccgg gacgcatcac
      841 agccacaggc cttcagtttg cccagggcac acgcattgga cacggcgtgc accacgc
//
