LOCUS       sequence-59719-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Mergenthaler P, Muselmann C, Sunwoldt J, Isaev NK, Wieloch T, 
            Dirnagl U, Meisel A, Ruscher K
  TITLE     A functional role of the cyclin-dependent kinase inhibitor 1 
            (p21(WAF1/CIP1)) for neuronal preconditioning.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Mar;33(3):351-5. doi: 
            10.1038/jcbfm.2012.213. Epub 2013 Jan 9.
  PUBMED    23299246
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2012.213"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-03"; volume: "33"; issue: "3"; pages: "351-5"
COMMENT     Sequence Label: pCAG-p21_WT
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..582
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(632..651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctaaccttct tctctttcct ctcctgacag gttggtgtac agtagcttcc accatggact
       61 cagatctcga gctcaagctt cgaattcatg tcagaaccgg ctggggatgt ccgtcagaac
      121 ccatgcggca gcaaggcctg ccgccgcctc ttcggcccag tggacagcga gcagctgagc
      181 cgcgactgtg atgcgctaat ggcgggctgc atccaggagg cccgtgagcg atggaacttc
      241 gactttgtca ccgagacacc actggagggt gacttcgcct gggagcgtgt gcggggcctt
      301 ggcctgccca agctctacct tcccacgggg ccccggcgag gccgggatga gttgggagga
      361 ggcaggcggc ctggcacctc acctgctctg ctgcagggga cagcagagga agaccatgtg
      421 gacctgtcac tgtcttgtac ccttgtgcct cgctcagggg agcaggctga agggtcccca
      481 ggtggacctg gagactctca gggtcgaaaa cggcggcaga ccagcatgac agatttctac
      541 cactccaaac gccggctgat cttctccaag aggaagccct aaggatccac cggtcgccag
      601 cggccgctag ctcgacatga taagatacat tgatgagttt ggacaaacca caactagaat
      661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtgaaatt
      721 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt t
//
