LOCUS       sequence-59734-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Burgo A, Sotirakis E, Simmler MC, Verraes A, Chamot C, Simpson JC, 
            Lanzetti L, Proux-Gillardeaux V, Galli T
  TITLE     Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in 
            neurite growth.
  JOURNAL   EMBO Rep. 2009 Oct;10(10):1117-24. doi: 10.1038/embor.2009.186. Epub
            2009 Sep 11.
  PUBMED    19745841
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2009.186"; journalName: "EMBO Rep"; date: "2009-10"; 
            volume: "10"; issue: "10"; pages: "1117-24"
COMMENT     Sequence Label: pEGFP VARP
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    152..273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(189..208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     243..262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(280..735)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(417..438)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     629..648
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        762..866
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(891..911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
       61 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
      121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      241 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
      301 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      361 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      421 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      481 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      541 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      601 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      661 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      721 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
      901 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      961 tctcaattag tcagcaacca
//
