LOCUS       sequence-59765-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Mack KL, Moroz OV, Moroz YS, Olsen AB, McLaughlin JM, Korendovych IV
  TITLE     Reprogramming EF-hands for design of catalytically amplified 
            lanthanide sensors.
  JOURNAL   J Biol Inorg Chem. 2013 Apr;18(4):411-8. doi: 
            10.1007/s00775-013-0985-5. Epub 2013 Feb 19.
  PUBMED    23420322
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00775-013-0985-5"; journalName: "J Biol Inorg Chem"; date:
            "2013-04"; volume: "18"; issue: "4"; pages: "411-8"
COMMENT     Sequence Label: pEXP5-NT-CuSeCat EE
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(492..512)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(525..542)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             561..583
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(614..633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(615..633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tcaccctttc acttcgctct catacgttgt
       61 acaaactctt catattctac ttgaccatca ccatcaatgt ctgcttccct aatcatttca
      121 tcaacttctt catctgttag cttctcccca cgatttgtca tcacaatacg aagttctgca
      181 gcttcaatgt aaccattacc atccttgtct tccatacgga acgcttctct aatttcttct
      241 tcgctatctg tatctttcat ttttcttgcc atcattgtca gaaactctgg gaagtcaatt
      301 gtgccattgc catcagcgtc tacttcattg atcatgtcct gtaattctgc ttctgtgggg
      361 ttctgaccaa gtgatctcat cacagtcccc aactcctttg tagttatagt accatcacca
      421 tccttgtcaa atagtgaaaa agcttctttg aattctgcaa tctgctcttc tgtcagttga
      481 tcagccataa gggactgaaa atacaggttt tcgccgctgc taccatgatg atgatgatga
      541 tgagaaccag acatggtata tctccttctt aaagttaaac aaaattattt ctagagggaa
      601 accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgggcag
      661 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
      721 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtg
//
