LOCUS       sequence-59833-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MPSTA
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggt
      121 gtcggcgcaa tgggtggcgg aggcgctgcg ggccccgcgc gctgggcagc ctctgcagct
      181 gctggacgct tcctggtacc tgccgaagct ggggcgcgac gcgcgacgcg agttcgagga
      241 gcgccacatc ccgggcgccg ctttcttcga catcgaccag tgcagcgacc gcacctcgcc
      301 ctacgaccac atgctgcccg gggccgagca tttcgcggag tacgcaggcc gcctgggcgt
      361 gggcgcggcc acccacgtcg tgatctacga cgccagcgac cagggcctct actccgcccc
      421 gcgcgtctgg tggatgttcc gcgccttcgg ccaccacgcc gtgtcactgc ttgatggcgg
      481 cctccgccac tggctgcgcc agaacctccc gctcagctcc ggcaagagcc aacctgctcc
      541 cgccgagttc cgcgctcagc tcgaccccgc cttcatcaag acctacgagg acatcaagga
      601 gaacctggaa tcccggcgct tccaggtggt ggactcccga gccactggca ggttccgcgg
      661 caccgagccc gagccccgag acggcattga acctggccac atcccaggta ccgtgaacat
      721 ccccttcaca gacttcctga gccaggaggg gctggagaag agccctgagg agatccgcca
      781 tctgttccag gagaagaaag tggacctgtc taagccactg gtggccacgt gtggctctgg
      841 cgtcacagcc tgccacgtgg cactaggggc ctacctctgc ggcaagccag acgtgcccat
      901 ctacgatggc tcctgggtgg agtggtacat gcgcgcccgg cccgaggatg tca
//
