LOCUS       sequence-59842-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJG4-5 converted Gateway Destination vector for yeast two-hybrid 
            prey vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY45
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(652..671)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
       61 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      121 gaatcaacta cttagatggt attagtgacc tgtagtcgac cgacagcctt ccaaatgttc
      181 ttcgggtgat gctgccaact tagtcgaccg acagccttcc aaatgttctt ctcaaacgga
      241 atcgtcgtat ccagcctact cgctattgtc ctcaatgccg tattaaatca taaaaagaaa
      301 taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc tacctattca
      361 tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa gaacaatttc acaactctta
      421 tacttttctc ttacaagtcg ttcggcttca tctggatttt cagcctctat acttactaaa
      481 cgtgataaag tttctgtaat ttctactgta tcgacctgca gactggctgt gtataaggga
      541 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
      601 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
      661 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
      721 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
      781 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
      841 aaccttaa
//
