LOCUS       sequence-60089-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TNKS2A
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..57)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(107..124)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             815..837
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    852..876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(876..895)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(877..895)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcagccttc tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc
       61 gagtgcggcc gcaagcttgt cgacggagct cgaattcgga tcctcaatgg tggtgatgat
      121 ggtgcgctcc atcgaccata ccttcaggcc tcataatctg gtaagtaatt aaatactcag
      181 gataagcctg ttctcctctg taaataacat attcagctaa tgctaggcca tttacactgg
      241 gcctaccagt gactgagtga tgacctggag gagaatgtgc cattttcatt gcactgaact
      301 gcaggaaaga ctttcccaag gttacccggc aaaagagcag ctgcctgtgg caaatgtaac
      361 aagatctgtc tttgtgaact ggacacccag tacctcctcc aattccatat acatattgat
      421 tgcttttgga agagttttca gcaaaataaa tgccagctcc aaacatacca cctatgtacg
      481 catgcctttc atcaaagcct ttgtggataa ttgcattcac aaaaggagac ccatgaaata
      541 gcattcgttc attggcatgg ttgtggtttt cttcagaaac ttcttttctc cggtgagtgt
      601 atctttccca tagtttcttg ttacaaacct tctgaatctt gagaatattg tatctgttga
      661 agattccacc tgcatgacct ccatctctgt gctctcgaac tgtactttgc atctcttcct
      721 ccacagactg aaactcttta tcatcaggag acagatctat aagaattgtt cccattaatt
      781 atctcctctt tgtgattcat gattcatatg tatatctcct tcttaaagtt aaacaaaatt
      841 atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc
      901 gcgggatcga gatctcgatc ctctac
//
