LOCUS       sequence-60110-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Anastasiou D, Poulogiannis G, Asara JM, Boxer MB, Jiang JK, Shen M, 
            Bellinger G, Sasaki AT, Locasale JW, Auld DS, Thomas CJ, Vander 
            Heiden MG, Cantley LC
  TITLE     Inhibition of pyruvate kinase M2 by reactive oxygen species 
            contributes to cellular antioxidant responses.
  JOURNAL   Science. 2011 Dec 2;334(6060):1278-83. doi: 10.1126/science.1211485.
            Epub 2011 Nov 3.
  PUBMED    22052977
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1211485"; journalName: "Science"; date: "2011-12-2-
            2"; volume: "334"; issue: "6060"; pages: "1278-83"
COMMENT     Sequence Label: pLHCX-Flag-mPKM1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             76..99
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccaagctt
       61 gttaacgcca ccatggacta caaagacgat gacgataaag caagggatcc actagtaacg
      121 gccgccagtg tgctggaatt catgccgaag ccacacagtg aagcagggac tgccttcatt
      181 cagacccagc agctccatgc agccatggct gacaccttcc tggaacacat gtgccgcctg
      241 gacattgact ctgcccccat cacggcccgc aacactggca tcatttgtac cattgggcct
      301 gcttcccgat ctgtggagat gctgaaggag atgattaagt ctggaatgaa tgtggctcgg
      361 ctgaatttct ctcatggaac ccatgagtac catgcagaga ccatcaagaa tgtccgtgaa
      421 gccacagaaa gctttgcatc tgatcccatt ctctaccgtc ctgttgcggt ggctctggat
      481 acaaagggac ctgagatccg gactggactc atcaagggca gcggcaccgc tgaggtggag
      541 ctgaagaagg gagccactct gaagatcacc ctggacaacg cttacatgga gaagtgtgac
      601 gagaacatcc tgtggctgga ctacaagaac atctgcaagg tggtggaggt gggcagcaag
      661 atctacgtgg acgatgggct catctcactg caggtgaagg agaaaggcgc tgacttcctg
      721 gtgacggagg tggagaatgg tggctccttg ggcagcaaga agggcgtgaa cctgccgggc
      781 gctgctgtgg atctccccgc tgtgtcggaa aaggacatcc aggacctgaa gtttggggtg
      841 gagcaggatg tggacatgg
//
