LOCUS       sequence-60121-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Gurtan AM, Lu V, Bhutkar A, Sharp PA
  TITLE     In vivo structure-function analysis of human Dicer reveals 
            directional processing of precursor miRNAs.
  JOURNAL   RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.
  PUBMED    22546613
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2012-06"; volume: "18(6)"; pages: "1116-22. Epub 2012 Apr 30"
COMMENT     Sequence Label: pCAGGS-Flag-hsDicer (K70A)
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..45
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctcgagatt gatctaccat ggactacaaa gacgatgacg acaagcttaa aagccctgct
       61 ttgcaacccc tcagcatggc aggcctgcag ctcatgaccc ctgcttcctc accaatgggt
      121 cctttctttg gactgccatg gcaacaagaa gcaattcatg ataacattta tacgccaaga
      181 aaatatcagg ttgaactgct tgaagcagct ctggatcata ataccatcgt ctgtttaaac
      241 actggctcag gggcgacatt tattgcagta ctactcacta aagagctgtc ctatcagatc
      301 aggggagact tcagcagaaa tggaaaaagg acggtgttct tggtcaactc tgcaaaccag
      361 gttgctcaac aagtgtcagc tgtcagaact cattcagatc tcaaggttgg ggaatactca
      421 aacctagaag taaatgcatc ttggacaaaa gagagatgga accaagagtt tactaagcac
      481 caggttctca ttatgacttg ctatgtcgcc ttgaatgttt tgaaaaatgg ttacttatca
      541 ctgtcagaca ttaacctttt ggtgtttgat gagtgtcatc ttgcaatcct agaccacccc
      601 tatcgagaaa ttatgaagct ctgtgaaaat tgtccatcat gtcctcgcat tttgggacta
      661 actgcttcca ttttaaatgg gaaatgtgat ccagaggaat tggaagaaaa gattcagaaa
      721 ctagagaaaa ttcttaagag taatgctgaa actgcaactg acctggtggt cttagacagg
      781 tatacttctc agccatgtga gattgtggtg gattgtggac catttactga cagaagtggg
      841 ctttatgaaa gactgctgat ggaattagaa gaagcactta attttatcaa tgattgtaat
      901 atatctgtac attcaaaaga aagagat
//
