LOCUS       sequence-60158-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PTGDSA
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             673..695
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    710..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(837..856)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg caagcttgtc gacggagctc gaattcggat cctcaatggt ggtgatgatg
      121 gtgcgcttgt tccgtcatgc acttatcggt ttggggcagg aagacaatgg tatcctctgt
      181 gaagccctgg gccttgcaga aggcggtgaa tttctccttt aactcagccc tgggggtctg
      241 ggttcggctg tagagggtgg ccatgcggaa gtcctcgcca gggcccttgc tgccctggct
      301 gtacagcagc gcgtactggt cgtagtcggt ctccaccact gacacggagt aggtgctgcc
      361 ccagtgggga ctccggtagc tgtaggagcc gagggacccc gcgggctgca gcagcatggt
      421 tcgggtctca cactggtttt tcctgaggaa ggtggaggtc aggttgaggc caccatccgt
      481 ggcaggggcc accacagact tgcacatgga caacgccgcc ttcttctccc ggagccagct
      541 cgagttggag gcgaggcccg cgctgaacca gcgccccagg aacttgtcct gctggaagtt
      601 gggctgcacg gagacctggg cctccggtgc cattaattat ctcctctttg tgattcatga
      661 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      721 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      781 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      841 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      901 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggc
//
