LOCUS       sequence-60380-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT12BA
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgggcgagg
      121 tggtcatgag ccaagccatc cagccggcgc acgccactgc gcgcggcgag ctgagcgcgg
      181 ggcagctgct caagtggatc gacaccaccg cctgcctggc ggctgagaaa catgctggag
      241 tttcctgcgt tacagcctca gtggatgaca tacagtttga ggagacagct agagttggac
      301 aagttataac catcaaagca aaagttacta gagcattcag cacaagcatg gagatcagta
      361 tcaaggtcat ggtacaggat atgctcactg gcattgagaa gcttgttagt gtggctttct
      421 ccacatttgt agccaaacca gttggaaaag aaaagattca tttaaaacca gtcacacttc
      481 taactgaaca agatcatgtg gaacataatc tggctgctga gagaaggaaa gttcgattac
      541 aacatgaaga tacctttaac aatttaatga aggaaagtag caaatttgat gatctcattt
      601 ttgatgaaga ggaaggagcg gtttccacaa ggggcacctc cgttcagagc attgaactgg
      661 tcctcccacc ccatgcaaac catcacggaa atacatttgg tggccagatt atggcgtgga
      721 tggagacagt ggctactatt tctgcaagcc gcctgtgttg ggctcatccc tttctgaagt
      781 ccgtagatat gtttaagttc cggggaccat ctacagttgg agatcgtctt gtcttcactg
      841 ccattgtcaa caatacattt cagacctgtg ttgaagttgg a
//
