LOCUS       sequence-60383-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP15A
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             773..790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(843..861)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      857..904
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgaatcttc
      121 ctgaacactg gactgacatg aatcatcagc tgttttgcat ggtccagcta gagccaggac
      181 aatcagaata taataccata aaggacaagt tcacccgaac ttgttcttcc tacgcaatag
      241 agaagattga gaggatacag aatgcatttc tctggcagag ctaccaggta aagaaaaggc
      301 aaatggatat caagaatgac cataagaata atgagagact cctcttccat gggacagatg
      361 cagactcagt gccatatgtc aatcagcacg gctttaatag aagttgtgct gggaaaaatg
      421 ctgtatccta tggaaaagga acctattttg ctgtggatgc cagttattct gccaaggaca
      481 cctactccaa gccagacagc aatgggagaa agcacatgta cgttgtgcga gtacttactg
      541 gagtcttcac aaagggacgt gcaggattag tcacccctcc acccaagaat cctcacaatc
      601 ccacagatct ctttgactca gtgacaaaca atacacgatc tccaaagcta tttgtggtat
      661 tctttgataa tcaggcttac ccagaatatc tcataacttt cacggcttga cagtaaaggt
      721 ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca
      781 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      841 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      901 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg a
//
