LOCUS       sequence-60399-e        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, 
            Pachigar K, Ludlow R, Khani SC, Smith WC
  TITLE     Expression characteristics of dual-promoter lentiviral vectors 
            targeting retinal photoreceptors and Muller cells.
  JOURNAL   Mol Vis. 2010 May 27;16:916-34.
  PUBMED    20517486
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Vis."; 
            date: "2010-05-27"; volume: "16"; pages: "916-34"
COMMENT     Sequence Label: pFIN-RK-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     232..252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1873..2106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2368..2485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2859..2868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2865..2867,2868..2870,2871..3584)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2865..2867,2868..2870,2871..3581)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2910..2931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3171..3190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3518..3539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3625..4213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3678..3698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4096..4107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4216..4232)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4217..4233)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5137..5361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5494..5513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6534..6551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6785..6804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8087..8106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8497..8515
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8566..8588)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8674..8812
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8727..8746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtggcccgag agctgcatcc ggagtatcta gatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgactga
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgc taatgggagt ttgttttggc
      181 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      241 gcggtaggcg tgtactctag aaggtctata taagcagagc tcgtttagtg aaccgtttat
      301 actacttatc tggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgctcaaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagtaaagcc agaggagatc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacaa tagcagtcct
      901 ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag ataagataga
      961 ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca caggaaacaa
     1021 cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa tggtacatca
     1081 ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga aggctttcag
     1141 cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac aagatttaaa
     1201 taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa aagagaccat
     1261 caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc ctattgcacc
     1321 aggccagatg agagaaccaa ggggaagtga catagcagga actactagca aattaagaga
     1381 acaatttgga aataataaaa caataatctt taagcaatcc tcaggagggg acccagaaat
     1441 tgtaacgcac agttttaatt gtggagggga atttttctac tgtaattcaa cacaactgtt
     1501 taatagtact tggtttaata gtacttggag tactgaaggg tcaaataaca ctgaaggaag
     1561 tgacacaatc acactcccat gcagaataaa acaatttata aacatgtggc aggaagtagg
     1621 aaaagcaatg tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg
     1681 gctgctatta acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg
     1741 aggaggcgat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1921 cgcacggtca atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca
     1981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     2041 gggcatcaaa cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     2101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     2161 tgctagttgg agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg
     2221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggctt gcggccggaa
     2341 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2461 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     2521 ggcggccgct agcatgccta ggtcgacccg ggatccgggc cccagaagcc tggtggttgt
     2581 ttgtccttct caggggaaaa gtgaggcggc cccttggagg aaggggccgg gcagaatgat
     2641 ctaatcggat tccaagcagc tcaggggatt gtctttttct agcaccttct tgccactcct
     2701 aagcgtcctc cgtgaccccg gctgggattt agcctggtgc tgtgtcagcc ccggtctccc
     2761 aggggcttcc cagtggtccc caggaaccct cgacagggcc cggtctctct cgtccagcaa
     2821 gggcagggac gggccacagg ccaagggcgg taccggtcgc caccatggtg agcaagggcg
     2881 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2941 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3001 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     3061 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     3121 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3181 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3241 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3301 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     3361 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     3421 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     3481 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3541 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
     3601 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3661 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3721 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3781 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3841 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3901 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3961 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4021 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4081 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4141 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4201 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     4261 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     4321 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     4381 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccag
     4441 tttcgagttg gcgcgcctgg gagctcacgg ggacagcccc ccccaaagcc cccagggatg
     4501 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     4561 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     4621 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     4681 gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca ggcgcgcctg
     4741 ggagctcacg gggacagccc cccccaaagc ccccagggat gtaattacgt ccctcccccg
     4801 ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc
     4861 ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc
     4921 ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaag
     4981 ctttaggctg aaagagagat ttagaatgac aggcgcgcca actcgaattc attgcagact
     5041 ttcactgaaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5101 tagggaaccc actgcttaag cctcaataaa gctcgactgt gccttctagt tgccagccat
     5161 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5221 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5281 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5341 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5401 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5461 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5521 tcgccacgtt cgccggctgt gcgggagaac ggagttctat tatgactcaa atcagtctcc
     5581 ccaagcattc ggggatcaga gtttttaagg ataacttagt gtgtaggggg ccagtgagtt
     5641 ggagatgaaa gcgtagggag tcgaaggtgt ccttttgcgc cgagtcagtt cctgggtggg
     5701 ggccacaaga tcggatgagc cagtttatca atccgggggt gccagctgat ccatggagtg
     5761 cagggtctgc aaaatatctc aagcactgat tgatcttagg ttttacaata gtgatgttac
     5821 cccaggaaca atttggggaa ggtcagaatc ttgtagcctg tagctgcatg actcctaaac
     5881 cataatttct tttttgtttt ttttttttta tttttgagac agggtctcac tctgtcacct
     5941 aggctggagt gcagtggtgc aatcacagct cactgcagcc tcaacgtcgt aagctcaagc
     6001 gatcctccca cctcagcctg cctggtagct gagactacaa gcgacgcccc agttaatttt
     6061 tgtatttttg gtagaggcag cgttttgccg tgtggccctg gctggtctcg aactcctggg
     6121 ctcaagtgat ccagcctcag cctcccaaag tgctgggaca accggggcca gtcactgcac
     6181 ctggccctaa accataattt ctaatctttt ggctaatttg ttagtcctac aaaggcagtc
     6241 tagtccccag gcaaaaaggg ggtttgtttc gggaaagggc tgttactgtc tttgtttcaa
     6301 actataaact aagttcctcc taaacttagt tcggcctaca cccaggaatg aacaaggaga
     6361 gcttggaggt tagaagcacg atggaattgg ttaggtcaga tctctttcac tgtctgagtt
     6421 ataattttgc aatggtggtt caaagactgc ccgcttctga caccagtcgc tgcattaatg
     6481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     6901 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8461 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8521 cgtcttcaag aactgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     8581 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     8641 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     8701 tagcggagtg tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     8821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     8881 ccagcgcggg gaggcagaga ttgcagtaag ctgagatcgc agcactgcac tccagcctgg
     8941 gcgacagagt aagactctgt ctcaaaaata aaataaataa atcaatcaga tattccaatc
     9001 ttttccttta tttatttatt tattttctat tttggaaaca cagtccttcc ttattccaga
     9061 attacacata tattctattt ttctttatat gctccagttt tttttagacc ttcacctgaa
     9121 atgtgtgtat acaaaatcta ggccagtcca gcagagccta aaggtaaaaa ataaaataat
     9181 aaaaaataaa taaaatctag ctcactcctt cacatcaaaa tggagataca gctgttagca
     9241 ttaaatacca aataacccat cttgtcctca ataattttaa gcgcctctct ccaccacatc
     9301 taactcctgt caaaggcatg tgccccttcc gggcgctctg ctgtgctgcc aaccaactgg
     9361 catgtggact ctgcagggtc cctaactgcc aagccccaca gtgtgccctg aggctgcccc
     9421 ttccttctag cggctgcccc cactcggctt tgctttccct agtttcagtt acttgcgttc
     9481 agccaaggtc tgaaactagg tgcgcacaga gcggtaagac tgcgagagaa agagaccagc
     9541 tttacagggg gtttatcaca gtgcaccctg acagtcgtca gcctcacagg gggtttatca
     9601 cattgcaccc tgacagtcgt cagcctcaca gggggtttat cacagtgcac ccttacaatc
     9661 attccatttg attcacaatt tttttagtct ctactgtgcc taacttgtaa gttaaatttg
     9721 atcagaggtg tgttcccaga ggggaaaaca gtatatacag ggttcagtac tatcgcattt
     9781 cagg
//
