LOCUS       sequence-60526-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH054
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(139..243)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
       61 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      241 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
      301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcccctcag cgcggccgct
      361 agcggatcct taaaaaacca tggccgcctc aaaaggagaa gaaacgacaa tgggtgttat
      421 caagccagac atgaagatca agcttaaaat ggagggtaat gttaatggtc acgccttcgt
      481 cattgaggga gagggtgaag gaaagccata cgatggaact aatactatta acttggaagt
      541 gaaggagggt gccccacttc cattctctta tgatatcctt actacagcct tcgcatatgg
      601 aaatcgagca ttcacaaaat accc
//
